Uveal melanoma is the most common intraocular tumor in adults. Despite advances in the accuracy of diagnosis, the ten-year mortality rate for patients has remained constant at approximately fifty percent. This indicates that a further understanding of the biological mechanisms behind this malignancy is required. / Our laboratory utilizes five human uveal melanoma cell lines (92.1, MKT-BR, OCM-1, SP-6.5, UW-1) that have a previously described metastatic potential from an animal model (MP). We used four methods to characterize the proliferation rate of these five cell lines. We also used a Matrigel invasion assay to assess the invasive ability of the same cell lines in response to the potential chemo-attractants: interleukin-6, Vascular Endothelial Growth Factor, and Fetal Bovine Serum. / From these results we were able to propose a novel classification system for our cell lines: high proliferation and high invasion/high MP, low proliferation and low invasion/low MP, and high proliferation and invasion/no MP. / From these results we were able to propose a novel classification system for our cell lines: high proliferation and high invasion/high MP, low proliferation and low invasion/low MP, and high proliferation and invasion/no MP.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.82296 |
| Date | January 2004 |
| Creators | Marshall, Jean-Claude |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Pathology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002199189, proquestno: AAIMR12507, Theses scanned by UMI/ProQuest. |
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