Background. Although Detrusor Hyperactivity (DH) with Impaired Contractility (IC) is a common urodynamic finding in elderly subjects, its pathogenesis remains unknown. Human detrusor biopsy studies indicate that subjects with DHIC exhibit ultrastructural evidence of both the dysfunction and degeneration patterns present in isolated DH and IC, respectively. Based on the known cellular effects of estrogen, we proposed a hypothesis that declines in ovarian hormone production could contribute to the pathogenesis of DHIC in elderly women. / Methods. In this thesis project, mature 3--14 month old female F-344 rats were studied 4 months after bilateral ovariectomy (OVx) or sham surgery. Detrusor structure was evaluated at this time point using light and electron microscopy, while classical muscle strip studies were used to measure the impact of OVx on detrusor muscle contractility. In an effort to identify estrogen-regulated proteins in the mammalian detrusor, known candidate proteins were screened using Western blotting, while identification of novel proteins was undertaken through proteomics, with two-dimensional gel protein resolution followed by microsequencing. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.31566 |
| Date | January 2000 |
| Creators | Zhu, Qing, 1960- |
| Contributors | Kuchel, George A. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Experimental Medicine.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001803224, proquestno: MQ70536, Theses scanned by UMI/ProQuest. |
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