Studies on normal and regenerated rat aortic endothelium at 1, 2, 3 and 4 weeks following segmental balloon denudation injury were carried out to evaluate: (1) the permeability to intravenously injected Evans blue (EB) and horseradish peroxidase (HRP) using en face aortic preparations and (2) the volume density of stress fibers by morphometry using thin section electron microscopy. The results of these studies indicated that: (1) the permeability of the regenerated endothelium to both EB and HRP was identical to normal endothelium at all time points studied and (2) stress fiber volume density significantly increased in regenerated endothelium at 1 week as compared to control, however, returned to and remained at normal value at and after 2 weeks following segmental balloon injury. These results are consistent with the view that structural and functional changes in regenerated vascular endothelium, if present, are transient in nature and the integrity of endothelial monolayer is eventually reestablished during the repair process that follows a single injury.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.59544 |
| Date | January 1990 |
| Creators | Cokay, Mehmet Sami |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Pathology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001233399, proquestno: AAIMM63681, Theses scanned by UMI/ProQuest. |
Page generated in 0.0017 seconds