Initiating the muscle differentiation pathway typically results in activation of muscle-specific genes previously maintained in a silenced state. One of the processes controlling changes in gene expression during differentiation is a global demethylation event. The mechanisms involved in this global hypomethylation are not fully understood. / Promoter methylation is one of the normal mechanisms inactivating gene expression. A single CpG site in the 5' flanking region of myogenin is reported to undergo demethylation during C2C12 differentiation. Considering the demethylase feature of MBD2b and its expression profile during C2C12 differentiation, I propose the hypothesis that MBD2b/demethylase is involved in C2C12 differentiation by demethylating the promoter of the myogenin gene as well as other genes involved in myogenic differentiation. / To test this hypothesis, I determined the consequences of up-regulation and down-regulation of MBD2b/demethylase in C2C12 cells. / The state of the myogenin promoter was also altered as determined using a probe recognizing a single HpaII site, which was previously reported to become demethylated during C2C12 differentiation. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.80861 |
| Date | January 2003 |
| Creators | Ren, Huiping |
| Contributors | Szyf, Moshe (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Pharmacology & Therapeutics..) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002141485, proquestno: AAIMQ98726, Theses scanned by UMI/ProQuest. |
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