A Cisplatin-resistant subline of a Human Ovarian cancer cell line, A2780 CP also exhibits cross resistance to 60Co gamma-radiation. Cellular Glutathione (GSH) levels were measured by the Tietze Spectrophotometric method. GSH levels in A2780 CP cells were approximately three time that of the parental A2780 cells. In cells depleted of GSH by L-Buthionine Sulfoximine (BSO), Cisplatin-resistance was significantly decreased in both cell lines. BSO had no effect on the radiation resistance of either cell lines. However treatment of cells with a combination of BSO and Dimethyl Fumarate (DMF) significantly decreased the radio-resistance in A2780 CP cells. The combination treatment had no effect on the radio-response of the parental cells. Ethacrynic acid (EA), an inhibitor of Glutathione S-Transferase (GST) activity had no effect on cellular GSH levels nor did it affect the Cisplatin or radiation resistance in either cell line. These results suggest that GSH levels are involved in Cisplatin and radiation resistance in these cells. However the mechanism of GSH involvement in radiation and Cisplatin-resistance appears to be different in the cell lines studied.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.21549 |
| Date | January 1998 |
| Creators | English, Michael Joseph. |
| Contributors | Lehnert, Shirley M. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Medical Radiation Physics.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001650108, proquestno: MQ50764, Theses scanned by UMI/ProQuest. |
Page generated in 0.002 seconds