We have used a functional in vitro assay to define several populations of B-cell and B-cell precursors normally present in the murine bone marrow. A multi-parameter analysis of cells (including size, polyclonal activation requirements and kinetics of recruitment to IgM secretion, membrane IgM expression, nylon-wool adherence properties and proliferative status) has permitted the resolution of up to 10 populations. Cells showing a delayed kinetics of development to plasma cells as compared to small B-lymphocytes were also found immature with respect to age-related markers. The appearance of characteristic markers (nylon wood adherence, membrane IgM) and functional properties (LPS-responsiveness) was traced within distinguishable populations of cells. On the basis of functional properties (AE-dependence, LPS-responsiveness and time of peak response) these 10 populations were grouped into 3 distinct subsets. From the major discontinuities in the kinetics of individual parameters and from changes in the quantitative expression of age-related markers, these subsets were sequentially related into the following scheme: / pre-B cells (--->) early B cells (--->) B-lymphocytes / Finally, a proliferative pathway was found within the early B cell compartment which parallels the post-mitotic maturation of the pre-B cell progeny.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68639 |
| Date | January 1982 |
| Creators | Deslauriers, Noëlla. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Medicine) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000157083, proquestno: AAINK60907, Theses scanned by UMI/ProQuest. |
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