The recently developed method of nuclear magnetic resonance spectroscopy allows for continuous monitoring of high energy phosphate compound (ATP and Phosphocreatine) and intracellular pH of tissues or whole organs. In other to better understand why ex-vivo hearts can be preserved for longer periods when perfused with oxygenated crystalloid solutions under hypothermic conditions, two groups of canine hearts were studied with nuclear magnetic resonance spectroscopy. / Group 1 = Canine hearts preserved for 4 hours by immersion into a 4$ sp circ$C saline solution. / Group 2 = Canine hearts preserved for 24 hours by continuous coronary perfusion with a modified oxygenated Krebs solution at 4$ sp circ$C. / The longer preservation of ATP and phosphocreatine, as well as the slower decrease of intracellular pH in Group II hearts are hypothesized to be the reasons why perfused hearts can be preserved for longer periods of time.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.56680 |
| Date | January 1992 |
| Creators | De Varennes, Benoit |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001306609, proquestno: AAIMM80466, Theses scanned by UMI/ProQuest. |
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