Differentiation between rejection and infection of lung allografts remains difficult. The effects of these two pathologic entities on the cytolytic activity of bronchoalveolar lavage (BAL) and peripheral blood lymphocytes (PBL) were investigated. Left lung allotransplantation was performed on sixteen mongrel dogs of which twelve were available for complete studies. All animals received cyclosporine A, azathioprine and prednisone for 2 weeks. Four grafts developed left lower lobe Gram negative pneumonia. The eight remaining recipients progressed gradually to severe rejection following acute reduction of immunosuppression. Cytolytic activity of blood and left lung BAL lymphocytes was quantitated by the natural killer (NK) and lectin dependent cell mediated cytotoxicity (LDCMC) assays. Two additional groups serving as controls were either given a ten day course of immunosuppressants or had right lower lobe pneumonia induced by transbronchial inoculation of gram negative bacteria. Immunosuppressed control animals showed significant depression of PBL and BAL lymphocyte LDCMC and NK activity. Similarly, BAL lymphocytes expressed very low LDCMC in normal allografts (2.8 $ pm$.8%). Once rejection developed and progressed, LDCMC became significantly higher (15.6 $ pm$ 2.2% and 52.7 $ pm$ 2.8% in moderate and severe rejection respectively). There was no detectable NK activity in rejecting lung allografts. BAL lymphocytes from infected allografts, on the other hand, showed an elevation of both NK and LDCMC activity (9.1 $ pm$ 1.1% and 14.6 $ pm$ 1.0% respectively). Similarly, bacterial pneumonia in control animals manifested an increase in NK and LDCMC activity in lung and blood. Peripheral blood lymphocytes of lung allograft recipients, however, had increased NK and LDCMC activity in both rejection and infection. LDCMC/NK activity ratio (LM/NK index) of lung lymphocytes was significantly higher in rejecting allografts (11.2 $ pm$ 1.0 and 12.4 $ pm$ 1.6 for moderate and sev
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.69539 |
| Date | January 1992 |
| Creators | Nguyen, Dao Minh |
| Contributors | Shennib, Hani (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001319507, proquestno: AAIMM87837, Theses scanned by UMI/ProQuest. |
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