Anemia is an almost invariable manifestation of chronic renal failure (CRF) and it often contributes substantially to the morbidity of the condition. In its uncomplicated form, this anemia is due primarily to reduced production of erythropoietin (EPO) by the diseased kidney. The present study was carried out in order to determine the relative role in the anemia secondary to CRF of EPO and insulin-like growth factor-1 (IGF-1), a recently recognized important regulatory factor of erythropoiesis in the normal physiological state. / A mouse model of CRF was employed in this investigation. Six weeks after the surgical induction of renal failure, the mice were characterized in terms of biochemical and hematological parameters which included the response to a 3-week treatment with recombinant human EPO (r-HuEPO). Additionally the kidneys, liver and bone marrow were harvested for the determination of the mRNA expression of EPO and IGF-1 as assessed by the reverse transcription polymerase chain reaction followed by Southern blotting. Normal mice and mice rendered anemic by phlebotomy were included in all experiments. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.69733 |
| Date | January 1993 |
| Creators | Zhang, Fenz |
| Contributors | Gagnon, Raymonde F. (advisor), Brox, Alan (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Physiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001351724, proquestno: AAIMM91848, Theses scanned by UMI/ProQuest. |
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