Heterogeneity is a ubiquitous finding in human tissues. Indeed, the cellular heterogeneity of a tumor may be viewed as a caricature of normal tissue that defines the tissue of tumor origin and prognosis. The heterogeneity of a simple tissue, bladder epithelium, has been studied with quantitative flow cytometry to measure light scattering properties and lectin binding to cells in normal and malignant tissue. It was confirmed that such quantitative assessments could be used to reveal relationships of normal tissue heterogeneity to malignant tissue heterogeneity which were relevant to both normal tissue development and tumor status. In addition, an indicator of DNA replication/cell proliferation (a monoclonal antibody for DNA cruciforms) was studied for future use in normal and tumor tissues to reveal various aspects of that functional heterogeneity. Fundamental methods for quantitative assessment and qualitative distribution of the initiation sites of DNA replication were established and applied to normal and malignant cell lines. Such functional analyses of heterogeneity hold the prospect of identification and characterization of tumor stem cell populations.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.74339 |
| Date | January 1989 |
| Creators | Ward, Glen Kielland |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Physiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001069300, proquestno: AAINN63697, Theses scanned by UMI/ProQuest. |
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