In this study we have shown that translocation of intestinal LPS into the portal and systemic circulatory systems during graft-versus-host disease (GVHD) is predictive of morbidity and mortality. Randomized C57BL/6 x AF1 (B6AF$ sb1$) mice were injected with C57BL/6 (B6) lymphoid cells and sacrificed at predetermined times after transplantation for bacterial lipopolysaccharide (LPS) tissue analysis. The liver, the spleen and the sera from some acute GVH reactive mice first tested positive for LPS from day 2, 4 and 16 post transplant, respectively. Total hepatic and splenic LPS in acute GVH reactive mice peaked, and LPS was first detected in the sera of these same animals, at a time coincident with the onset of mortality. These results show that LPS is present to initiate tumour necrosis factor alpha (TNF-$ alpha$) release from interferon gamma (IFN-$ gamma$) primed macrophages, resulting in the manifestations of acute GVHD.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68245 |
| Date | January 1993 |
| Creators | Price, Kursteen S. (Kursteen Salter) |
| Contributors | Lapp, W. S. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Physiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001397533, proquestno: AAIMM94503, Theses scanned by UMI/ProQuest. |
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