Transplantation of the small intestine is technically feasible, and the only potentially curative method for patients with short gut syndrome. However this procedure is still infrequent, partially because there is still no reliable method to preserve the small bowel (SB) for a reasonable period of time between removal from the donor and transplantation. In search of a suitable medium for SB preservation, we evaluated different solutions which have been successfully used for preservation of other human organs (Eurocollins (EC), University of Wisconsin (UW) and lactated Ringer's (LR)) and tried to improve their effectiveness by adding superoxide dismutase and catalase or verapamil. The adequacy of preservation was assessed by evaluating the physiological properties of the intestine in vitro, using either the rat syngeneic model of intestinal transplantation, or human intestine obtained from organ donors. / LR is a simple, inexpensive and universally available solution, and when supplemented with verapamil, it was as effective as the more complex EC and UW as a protectant against ischemic damage during cold storage of rat ileum. Studies on human intestine validated the rat as an experimental model since the relative effectiveness of the different solutions was similar, however, the human bowel appeared more vulnerable to ischemic and mechanical damage. The results indicate that creation of an effective preservation solution for the small intestine should be possible through appropriate modification of currently available preparations.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.23915 |
| Date | January 1995 |
| Creators | Lubin, Alexandre |
| Contributors | Zorychta, E. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Pathology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001481361, proquestno: MM12230, Theses scanned by UMI/ProQuest. |
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