The properties of (beta)-N-acetylhexosaminidase (HEX EC 3.2.1.30 and 3.2.1.53) in canine serum during endotoxin induced experimental shock were investigated. / In normal canine serum, HEX activity is very low and the enzyme exists in two major isoenzymic forms: a predominant acidic form (HEX A) and a basic form (HEX B). Both HEX A and HEX B as well as a third isoenzyme HEX A('1) are also present in canine tissues. Only HEX A and HEX B are present in the urine. / "Shocked" serum contains very high levels of total HEX activity with increased activity of both isoenzymes HEX A and HEX B, as well as the appearance of an additional isoenzyme (HEX A('1)). In shock, HEX A('1) accounted for over 28% of total serum HEX activity. HEX A was diminished while HEX B was significantly higher. / HEX A('1) differs in physical and enzymatic properties from HEX A and HEX B of normal serum. It is suggested that HEX A('1) is released from tissues during shock. / Methylprednisolone prevented in vitro the intracellular activation of HEX, and in vivo prevented the increase in serum HEX activity during shock.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.68536 |
| Date | January 1980 |
| Creators | Baraya-Mil, Hamidou Stephen. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Experimental Surgery) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000102960, proquestno: AAINK51864, Theses scanned by UMI/ProQuest. |
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