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Early humoral changes in the lung allograft

The effects of ischemia/reperfusion injury on humoral changes in canines that underwent left lung allotransplantation was compared to autotransplantation (n = 10 each). Cytokines IL-2, TNF-a and IFN-g were measured in bronchoalveolar lavage (BAL) and plasma samples at 1, 4, 24 hours and 1 week postoperatively. In the allograft there was an early postoperative increase in all cytokines in BAL which decreased after 24 hours. The same trend was seen for IL-2 in the autograft. In contrast, TNF-a and IFN-g levels in the autograft remained unchanged. Using immunohistochemical staining techniques, MHC II antigen expression was observed on lung allograft bronchial epithelium which was less intense in the autograft. Hematoxylin and eosin staining of lung biopsies revealed early evidence of lung injury and also grade 1-2 rejection after 1 week in the allograft. Injury was not as severe in the autograft. We conclude that a temporary elevation of cytokines early after allotransplantation is partly due to ischemia/reperfusion injury and graft allogenicity. This early cytokine release may play an important role in the development of early graft dysfunction and rejection.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.69540
Date January 1992
CreatorsSerrick, Cyril James
ContributorsShennib, Hani (advisor)
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageMaster of Science (Division of Surgical Research.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 001323921, proquestno: AAIMM87838, Theses scanned by UMI/ProQuest.

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