The terminal events leading to periprosthetic osteolysis are multifactorial and attempts to modulate this process after the stage of modulator release have been futile. Cell death at the osteolytic interface of failed total hip arthroplasties (THAs) may occur via one of two modes---Apoptosis (programmed cell death/cellular suicide) vs. Necrosis. Apoptosis (PCD) is an active form of cell death that results in---among other things---orderly fragmentation of DNA and specific protein synthesis. The purpose of this study was to determine the mode of cell death at this interface. TdT-mediated dUTP nick end-labeling (TUNEL) stain and immunodetection of terminin protein were used to identify apoptosis. / Our studies demonstrated that 31% (range 7--56%) of cells of pseudomembrane were positive for TUNEL stain. Moreover, the presence of apoptosis was the same at both the femoral and acetabular sides of the prostheses. In addition, pseudomembrane (PM) specimens stained for the 30 kDa terminin protein, an apoptosis-specific protein. The results demonstrate that apoptosis is occurring at the PM-bone interface of THAs suggesting that apoptosis, related events may be associated with periprosthetic osteolysis.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.21596 |
| Date | January 1998 |
| Creators | Lisbona, Allan Elie. |
| Contributors | Huk, Olga (advisor), Zukor, David (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001650194, proquestno: MQ50823, Theses scanned by UMI/ProQuest. |
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