Identifying the molecular and genetic pathways important for heart development and deciphering the causes of CHD are still a challenging puzzle. A newly identified piece of this puzzle is KLF13, a member of the Krüppel-like family of zinc-finger proteins, was found to be important for atrial septation and ventricular trabeculation of Xenopus embryos. The protein is expressed predominantly in the heart, binds evolutionarily-conserved regulatory elements on cardiac promoters, and activates cardiac transcription.
In this study we examined KLF13 mechanism of action by investigating its transcriptional activity on the ANF promoter using a deletion/mutagenesis approach. We reported the identification of a new synergistic partnership between KLF13 and the individual cardiac transcription factors TBX5, NKX2.5, PEX1, and CATF1. Also, we localized KLF13’s transcriptional activation domain, the nuclear localization region/zinc-fingers, and the DNA binding zinc-fingers. This study will provide insight into the contribution of KLF13 to the development of CHDs.
Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OOU.#10393/20154 |
Date | 12 August 2011 |
Creators | Darwich, Rami |
Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
Language | English |
Detected Language | English |
Type | Thèse / Thesis |
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