Identifying the molecular and genetic pathways important for heart development and deciphering the causes of CHD are still a challenging puzzle. A newly identified piece of this puzzle is KLF13, a member of the Krüppel-like family of zinc-finger proteins, was found to be important for atrial septation and ventricular trabeculation of Xenopus embryos. The protein is expressed predominantly in the heart, binds evolutionarily-conserved regulatory elements on cardiac promoters, and activates cardiac transcription.
In this study we examined KLF13 mechanism of action by investigating its transcriptional activity on the ANF promoter using a deletion/mutagenesis approach. We reported the identification of a new synergistic partnership between KLF13 and the individual cardiac transcription factors TBX5, NKX2.5, PEX1, and CATF1. Also, we localized KLF13’s transcriptional activation domain, the nuclear localization region/zinc-fingers, and the DNA binding zinc-fingers. This study will provide insight into the contribution of KLF13 to the development of CHDs.
Identifer | oai:union.ndltd.org:uottawa.ca/oai:ruor.uottawa.ca:10393/20154 |
Date | January 2011 |
Creators | Darwich, Rami |
Contributors | Nemer, Mona |
Publisher | Université d'Ottawa / University of Ottawa |
Source Sets | Université d’Ottawa |
Language | English |
Detected Language | English |
Type | Thesis |
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