The study was conducted to investigate the effects of: (i) varying the level of supply of the heavy metals iron, manganese, copper and zinc. (ii) varying the ratio between different pairs of metals in the medium. In particular the iron:manganese and zinc:copper ratios were studied. Initially the two fungi Aspergillus niger van Tieghem, (variety and strain) and Penicillium notatum Westling, were used. Penicillium notatum Westling was subsequently discarded, in favour of Penicillium glancum Link, because it did not sporulate freely in liquid culture. The fungi were grown in controlled nutrient solutions, and during the course of tho growth and development, the form and sporulation of the felts was noted. After a period of growth, the felts were removed, dried and weighed. The pH of the liquor was measured. the results were studied to determine the effect of varying levels of supply of the heavy metals, and of the varying heavy metal ratios in the culture solution. In the investigation of the effect of varying the level of supply of individual heavy metals, optimum concentrations were demonstrated for copper and manganese. Increaning the concentration of pairs of heavy metals cimultaneously was found to influence the appearance and degree of symptoms of toxicity. Cultures of Aspergillus and Penicillium were found to be able to tolerate concentrations of copper, in particular, considerably greater than the observed optima, when zinc was present in equal concentration. Citric acid, and subsequently ethylene- diaminetetra-acetic acid, were used as chelating agents, in order to prevent the precipitation of the metals in the culture solution during autoclaving. It was found that the use of chelating agents markedly reduced symptoms of toxicity. There was no conclusive evidence that the iron:manganese ratio in the culture medium was an important factor in the growth and development of cultures of Aspergillus and Penicillium. However there is considerable evidence that in cultures of Penicillium, the zinc:copper ratio in the medium is of some importance in the determination of the dry weight yield trends. This effect was not demonstrated in cultures of Aspergillus.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:rhodes/vital:4268 |
| Date | January 1965 |
| Creators | Breen, C M |
| Publisher | Rhodes University, Faculty of Science, Botany |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis, Masters, MSc |
| Format | 233 p., pdf |
| Rights | Breen, C M |
Page generated in 0.0023 seconds