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Transfer of chlorsulfuron resistance from tobacco to birdsfoot trefoil (Lotus corniculatus) by asymmetric somatic hybridization

A method was developed for rapid plant regeneration from protoplasts of birdsfoot trefoil (Lotus corniculatus cv. Leo) using in vitro cotyledons and a new enzyme formula. Protoplasts of a transgenic Nicotiana tabacum cv. Xanthi line KCR were isolated from leaves, in vitro shoots, and calli by an enzyme formula consisting of 0.5 % Cellulase R-10 and 0.05 % Pectolyase Y23 with either 0.4 M sucrose or 0.5 M mannitol as the osmoticum. Nine asymmetric fusion experiments were conducted between iodoacetate inactivated birdsfoot trefoil and irradiated transgenic tobacco protoplasts. The fusion products underwent several divisions but no visible colonies were obtained. Resistance analyses revealed the inactivation of the herbicide resistant gene in the transgenic tobacco calli. A putative chlorsulfuron resistant line has been obtained by in vitro selection from birdsfoot trefoil cotyledonous protoplasts. Their regenerants had normal ploidy, and expressed improved tolerance when sprayed with chlorsulfuron at the rate of 30 g/ha.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.41010
Date January 1992
CreatorsVessabutr, Suyanee
ContributorsGrant, W. F. (advisor)
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageDoctor of Philosophy (Department of Plant Science.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 001324229, proquestno: NN87847, Theses scanned by UMI/ProQuest.

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