Animals who live in the high-altitude environment are faced with the harsh conditions of low oxygen availability. This can make it difficult to perform daily tasks that require aerobic metabolism. However, deer mice (Peromyscus maniculatus) have evolved physiological adaptions that allow populations to survive in the high-altitude environment. One activity important for survival is aerobic locomotion and the rapid recovery of muscle metabolism after a bout of exercise. Hypoxia acclimated high-altitude mice have a greater reliance on carbohydrates to power exercise than low altitude mice and show a significant depletion of muscle glycogen. However, it is unclear how quickly after exercise is finished that these mice can replenish muscle glycogen stores. The gastrocnemius muscle of high-altitude deer mice has a more aerobic phenotype and a greater capacity to oxidize lipids than in low altitude mice. This suggests that high altitude mice may recover more quickly from exercise than their lowland counterparts due to a greater capacity to power glycogen replenishment through aerobic metabolism using the lipids stored in muscle as intramuscular triglycerides (IMTG). Using low- and high-altitude native deer mice born and raised in common lab conditions and acclimated to chronic hypoxia, I determined changes in oxygen consumption (VO2) following aerobic exercise and sampled skeletal muscle at various time points during recovery to examine changes in key metabolites, including glycogen and IMTG. I found there was depletion in glycogen stores during exercise in lowlanders and glycogen did return to resting levels following 90 minutes of recovery. In contrast, IMTG’s did not significantly change with exercise or during the recovery period in either population. These data suggest that muscle recovery from aerobic exercise may be influenced by altitude ancestry in deer mice. / Thesis / Master of Science (MSc)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/28746 |
| Date | January 2023 |
| Creators | Dessureault, Lauren |
| Contributors | McClelland, Grant, Biology |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
| Type | Thesis |
Page generated in 0.002 seconds