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Technologie pěstování ostropestřece mariánského (Silybum marianum) ve vztahu ke kvalitě produktu a jeho zpracování / Cultivation Technology of Milk Thistle (Silybum Marianum)in Relation to the Product Quality and its Processing

Silybum marianum has been a plant known for millenniums thanks to its pharmacological effects especially in fields of liver, gallbladder or even colon cancer treatment. A complex of effective components is called silymarin. Its amount and structure in milk thistle seeds are very important. That{\crq}s why there{\crq}s a tendency to develop methods which could increase silymarin quantity together with silymarin quality in this herb. This was also the aim of this thesis. One of the possibilities to level up the content of effective components in drugs is to bring this plant in stress. There were realized two ground-plot experiments. The stress agens was acetatonsalicylic acid (ASA) of different concentrations applicated on leaves of these plants, concretely in the concentrations of 10-5 mol.l-1, 10-4 mol.l-1, 10-3 mol.l-1. In the case of the application ASA concentration 10-3 mol.l-1 there was proved an effective action. The increase of effective components in seeds reached approximately 116,5 % ratio compared with control application of distilled water on the Silybum marianum leaves. The silymarin complex was determined by High Performance Liquid Chromatography. The preparation of the extract was practised in different ways {--} using ethyl alcohol extract and distilled water in combination with various temperatures of extracts storage. These methods were compared one another. As the best one has been turned out to be using 60% ethanol concentration for the duration of 96 hours in the storage temperature 20°C.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:51272
Date January 2009
CreatorsGRAMANOVÁ, Hana
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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