The production of cereal crops is essential to secure a future that feeds
the continuously growing population. Rust fungi reduce host fitness by feeding on
their living tissue and interfere with the global production of crops. Cereal rusts,
like Puccinia hordei (the causal agent of barley leaf rust) and Puccinia triticina
(the causal agent of wheat leaf rust), have a narrow host range and colonize only
one particular species. The most durable type of resistance, non-host resistance
(NHR), is the immunity of an entire plant species to all strains of a pathogen
species. Exploring the genetics of NHR has proven to be challenging because
most interspecific hybrids are infertile. Previously, barley Rphq2 and Rph22,
which encode orthologous lectin receptor-like kinases (LecRKs), were
transformed into an experimental barley line, Golden SusPtrit, and showed
resistance against adapted and non-adapted leaf rust species. We used these
transgenic barley lines in the current project to explore the effect of the LecRKs
on four wheat leaf rust (P. triticina) isolates. We used the settling tower method to
inoculate four isolates of P. triticina on Rphq2 and Rph22 transgenic families. We
found that most transgenic families showed an increase in resistance compared
to the non-transgenic control 750-E1. By measuring the infection frequency of the
infections, we identified that P. triticina isolates 93012 and 95012 had opposite
virulence effects on two barley families, Rphq2-E5 and Rph22-E2A. Although the
expression levels of Rphq2 and Rph22 followed an induction trend, we did not
find significant differences between the isolates. We conclude that resistance
mediated by Rphq2 and Rph22 against P. triticina isolates does not involve an
isolate-specific component. Thus, we propose investigating differences between
rust species to further explore the molecular aspect of non-host resistance.
| Identifer | oai:union.ndltd.org:kaust.edu.sa/oai:repository.kaust.edu.sa:10754/669516 |
| Date | 05 1900 |
| Creators | Alburi, Dona |
| Contributors | Krattinger, Simon G., Biological and Environmental Sciences and Engineering (BESE) Division, Blilou, Ikram, Rayapuram, Naganand |
| Source Sets | King Abdullah University of Science and Technology |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Rights | 2021-06-09, At the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis became available to the public after the expiration of the embargo on 2021-06-09. |
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