Toward the next generation of nerve guidance conduits (NGCs), novel biomaterials and
functionalization concepts are required to address clinical demands in peripheral nerve regeneration
(PNR). As a biological polymer with bioactive motifs, gelatinous peptides are promising building
blocks. In combination with an anhydride-containing oligomer, a dual-component hydrogel system
(cGEL) was established. First, hollow cGEL tubes were fabricated by a continuous dosing and
templating process. Conduits were characterized concerning their mechanical strength, in vitro
and in vivo degradation and biocompatibility. Second, cGEL was reformulated as injectable shear
thinning filler for established NGCs, here tyrosine-derived polycarbonate-based braided conduits.
Thereby, the formulation contained the small molecule LM11A-31. The biofunctionalized cGEL filler
was assessed regarding building block integration, mechanical properties, in vitro cytotoxicity, and
growth permissive effects on human adipose tissue-derived stem cells. A positive in vitro evaluation
motivated further application of the filler material in a sciatic nerve defect. Compared to the empty
conduit and pristine cGEL, the functionalization performed superior, though the autologous nerve
graft remains the gold standard. In conclusion, LM11A-31 functionalized cGEL filler with extracellular
matrix (ECM)-like characteristics and specific biochemical cues holds great potential to support PNR.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:88747 |
| Date | 21 December 2023 |
| Creators | Kohn-Polster, Caroline, Bhatnagar, Divya, Woloszyn, Derek J., Richtmyer, Matthew, Starke, Annett, Springwald, Alexandra H., Franz, Sandra, Schulz-Siegmund, Michaela, Kaplan, Hilton M., Kohn, Joachim, Hacker, Michael C. |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1104, 10.3390/ijms18051104 |
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