The purpose of this study was to analyze the frequency distributions of alleles at the 3$ sp prime$HVR (hypervariable region) and 5$ sp prime$HVR, two highly polymorphic regions on chromosome 16p. About 300 DNA samples from individuals of East Asian, French Canadian, Greek, Italian, Jewish and Middle Eastern origin were analyzed by hybridization to probes for the 3$ sp prime$HVR and 5$ sp prime$HVR. / The distributions of alleles at both loci are skewed with the long tail towards the larger alleles. The observed heterozygosity at the 3$ sp prime$HVR locus for 281 individuals was 0.91, ranging from 0.85 in the Jewish group to 1.00 among French Canadians and East Asians. Statistical analysis demonstrated significant variation among some of the ethnic groups. / The observed heterozygosity at the 5$ sp prime$HVR locus in 225 individuals was 0.75. Heterozygosity varied from 0.91 in East Asians to 0.61 of Middle Eastern samples studied. 28% of samples also display a RsaI site polymorphism near the 5$ sp prime$HVR locus. / Genetic distance analysis demonstrated that the largest distance at these two loci exists between the Jews and East Asians (D = 0.119). / Both the 3$ sp prime$HVR and the 5$ sp prime$HVR are extremely variable in all the populations studied, and thus will serve as informative markers for chromosome 16p for clinical as well as population studies.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.59409 |
| Date | January 1989 |
| Creators | Marshall-Shapiro, Adele H. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001067497, proquestno: AAIMM63584, Theses scanned by UMI/ProQuest. |
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