Prostate cancer is a public health concern, particularly to the African-American community. African-American men are disproportionately affected and experience prostate cancer rates about 60% more often than white Americans. It is the second leading cancer in men and it is second only to lung cancer in cancer related deaths. This is a significant public health problem. This paper will focus on the gene Interleukin 6 signal transducer isoform 1 (IL6ST), on chromosome 5q11.2, with polymorphisms relating to gp130 [rs 3730293 and rs 3729960]; both single nucleotide polymorphisms result in an amino acid change. The change is Glycine to Arginine for rs 3729960 which is located in exon 14 and Isoleucine to Threonine for rs 3730293 which is located in exon 10. Human IL-6 signaling requires the 80kDa IL-6R receptor, which is responsible for IL-6 specificity and the 130kDa glycoprotein, gp130, the signal transduction subunit for signal transduction to occur. IL-6 acts through its receptors which are polymorphic, and subsequently may cause it to have different functionality. A population based case/control study was conducted to evaluate the influence of gp130 snps on prostate cancer risk in an Afro-Caribbean male population of Tobago. This population was chosen because of the high prevalence of prostate cancer. A subset of 1000 samples taken from a study done by Bunker et al, was genotyped by PCR and fluorescence polarization methods. Statistical analysis yielded all estimated allele frequencies to be in Hardy Weinberg Equilibrium. Chi square analysis of the cases and controls yielded no significant association of the gp130 snps and prostate cancer risk.
| Identifer | oai:union.ndltd.org:PITT/oai:PITTETD:etd-06232006-175315 |
| Date | 08 August 2006 |
| Creators | Roman, Lalicia N. |
| Contributors | Dr. Stephen Thomas, Dr. M. Michael Barmada, Dr. Robert Ferrell |
| Publisher | University of Pittsburgh |
| Source Sets | University of Pittsburgh |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.pitt.edu/ETD/available/etd-06232006-175315/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University of Pittsburgh or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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