Hyaluronic acid is an organic polysaccharide with a wide range of uses in medical and cosmetic industries due to its physiological properties. Crosslinked hyaluronic acid is a commonly used filler agent because of its water retention capabilities. N-deacetylation can be performed to enable new derivatives of hyaluronic acid. Both chemical and enzymatical approaches were investigated in this literature study to find methods retaining a high molecular weight product. Chemical N-deacetylation of hyaluronic acid has significant challenges with being treated by acid or base while both preventing degradation and maintaining its molecular weight. The method that seems the most promising is treating hyaluronic acid with hydroxylamine. Another method is enzymatic N-deacetylation. It was found that an enzyme N-deacetylated hyaluronic acid in female breast skin from 69-year-olds and above. The isolated enzyme had molecular weights ranging from 63 kDa to 79 kDa. Another enzyme that was produced recombinantly proved to be efficient since it retained high molecular weight and had a degree of deacetylation of 10.1 %. Today there exists only a few methods for crosslinking deacetylated hyaluronic acid. However, for chitosan, there are multiple methods available for crosslinking. Since it uses similar reactions that could be applicable to that of deacetylated hyaluronic acid. Reacetylation of the free amino groups has proven to be possible after crosslinking with a simple and cheap method resulting in an almost complete reacetylation. NMR proved to be an adequate method for analyzing the degree of deacetylation and higher-order structures. HPLC-UV spectroscopy may be used to increase the credibility of the analysis.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-503554 |
| Date | January 2023 |
| Creators | Mardini, Sima, Björk, Hanna, Möller, Marcus, Lagergren, Carl, Samuelsson, Oscar |
| Publisher | Uppsala universitet, Institutionen för materialvetenskap |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | MATVET-K ; 23004 |
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