The main aim of this study was to investigate biological hydrogen production from sucrose and molasses by purple non-sulphur bacteria (PNS). The hydrogen production capacities of four different PNS bacteria (Rhodobacter capsulatus (DSM 1710), Rhodobacter capsulatus YO3 (Hup-), Rhodopseudomonas palustris (DSM 127) and Rhodobacter sphaeroides O.U.001 (DSM 5864)) were tested on sucrose and molasses. The photobiological hydrogen production were performed in 50 ml and 150 ml small scale photobioreactors, in batch mode. The produced hydrogen quantities, bacterial growth profiles and pH of the media were recorded through the
photobiological hydrogen production processes. Organic acids and sucrose consumption rates were determined by HPLC during the experiments. The maximum hydrogen productivitiy of 0.78 (mmol/lc.h) and 0.55 (mmol/lc.h) was obtained by R. palustris (DSM 127) on sucrose and molasses, respectively. Secondly, co-cultivation of these bacterial strains was studied. The maximum hydrogen productivity by co-cultivation of R. sphaeroides O.U.001 (DSM 5864) and R. palustris (DSM 127) was found as 1.0 (mmol/lc.h).
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12614107/index.pdf |
| Date | 01 February 2012 |
| Creators | Sagir, Emrah |
| Contributors | Sagir, Emrah |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | Access forbidden for 1 year |
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