The biocatalysis of a crude chlorophyllase extract, obtained from the biomass culture of Phaeodactylum tricornutum, in neat organic solvent media was investigated. The addition of selected excipients, including crown ether (enzyme:crown ether, 135:1--2.7:1, w/w), dextran (enzyme:dextran, 1:2--1:0.25, w/w), Span 40 and Span 60 (enzyme:Span, 1:2, w/w) and sodium bis (2-ethylhexyl) sulfosuccinate (enzyme:AOT, 1:12.6, w/w), to the crude solid enzyme preparation decreased the chlorophyllase activity. The effects of selected parameters, including solvent hydrophobicity (Log P, 2.40--4.45), initial water activity (aw, 0.44--0.97), agitation speed (0--200 rpm), reaction temperature (25--45°C) and enzyme concentration (1.67--5.3 mg solid enzyme/mL) on chlorophyllase activity, were investigated using a crude solid enzyme. The experimental findings showed that the highest chlorophyllase specific activity of 362.4 nmol hydrolyzed chlorophyll/g solid enzyme/min and bioconversion yield of 90.7% were obtained with the hexane/octanone mixture (98.7:1.3, v/v), aw of 0.90, agitation speed of 200 rpm, reaction temperature of 35°C and enzyme concentration of 3.33 mg solid enzyme/mL.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.81263 |
| Date | January 2004 |
| Creators | Arriagada Strodthoff, Paula |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Food Science and Agricultural Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002173218, proquestno: AAIMR06373, Theses scanned by UMI/ProQuest. |
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