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Simple Pretreatment of Arundo Donax and Enzymatic Conversion of Cellulosic Materials to Glucose

Arundodonax (Giant reed Plant) contains cellulose, hemicellulose and lignin and considered as a biomass resources for biofuels. Cellulose is a polymer of several d-glucose linked units coupled with beta-1, 4 glycosidic bonds. The lignin must be broken down to obtain cellulose.Brown and white rot fungusbreak down lignin through a fenton mechanism using hydroxyl radicals. Current work explores degradation of cellulose byisolating microbial communities followed by inoculating 1% carboxymethyl cellulose (CMC) or arundodonax in nutrient media. The microbes demonstrate long-term viability using CMS or arundodonax the sole carbon source.Pretreatment with microbes result in enhanced enzymatic hydrolysis at 50 °C using commercial cellulase over time. The simple dinitrosalicylic acid assay method quantifies glucose, the main product of enzymatic hydrolysis.

Identiferoai:union.ndltd.org:ETSU/oai:dc.etsu.edu:asrf-1480
Date12 April 2019
CreatorsFatunwase, Akintayo
PublisherDigital Commons @ East Tennessee State University
Source SetsEast Tennessee State University
Detected LanguageEnglish
Typetext
SourceAppalachian Student Research Forum

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