Production of hypericine in explantate cultures of Hypericum perforatum Pavel Sazama Diploma thesis Charles university in Prague, Faculty of Pharmacy in Hradec Králové, Pharmacy Key words: St. John's Wort, precursors, hypericine, flavonoids, acetate, cinnamic acid, cinnamate, tyrosine, shikimic acid The goal of this work was to affect the production of hypericin (naphto-dianthrone), hyperoside and quercitrin (flavonoids) in the suspensional Hypericum perforatum explantate cell cultures. The method of precursor feeding was used. The precursors of naphtodianthrones and flavonoids were added into the medium in final concentrations 10 mg.l-1, 50 mg.l-1 and 100 mg.l-1. Samples were taken after 72 and 168 hours after adding the precursor. Potassium acetate, cinnamic acid, sodium cinnamate, tyrosine and shikimic acid were used as precursors. Cultures were cultivated on the Murashige and Skoog medium with the addition of the growth stimulator α-NAA. Concentration of hypericin, hyperosid and quercitrin was measured by HPLC analysis. The highest influence on the production of hypericin in cultures was detected after adding of tyrosine. The concetration of hypericin in cells raised in this experiment from 0,003% (control culture) to 0,03% (culture with tyrosine concen-tration of 100 mg.l-1). This...
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:345275 |
| Date | January 2016 |
| Creators | Sazama, Pavel |
| Contributors | Martin, Jan, Kašparová, Marie |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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