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Effect of hyperkalemia and ischemia on large conductance calcium-activated potassium channels in porcine coronary arterial smooth muscle: relevance to cardioplegic arrest. / 高鉀和缺血對豬冠狀動脈平滑肌大電導鈣激活鉀通道的影響--與心臟手術的相關性 / Gao jia he que xue dui zhu guan zhuang dong mai ping hua ji da dian dao gai ji huo jia tong dao de ying xiang -- yu xin zang shou shu de xiang guan xing

Han, Jianguo. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 66-76). / Abstracts in English and Chinese. / Declaration --- p.i / Acknowledgement --- p.□ / Publication --- p.□ / Abstract (English) --- p.□xi / Abstract (Chinese) --- p.□ / Abbreviations --- p.ix / List of figures / tables --- p.x / Chapter Chapter 1. --- General Introduction / Chapter 1.1 --- Role of vascular smooth muscle cells in the control of coronary circulation --- p.1 / Chapter 1.1.1 --- Potassium channels in the coronary smooth muscle cells --- p.2 / Chapter 1.1.1.1 --- Voltage -dependent potassium (Kv) channels --- p.3 / Chapter 1.1.1.2 --- Inward rectifier K+ (Kir) channels --- p.4 / Chapter 1.1.1.3 --- ATP-sensitive potassium (Katp) channels --- p.4 / Chapter 1.1.2 --- BKCa channels in the regulation of vascular function --- p.6 / Chapter 1.1.2.1 --- The structure of BKCa channels --- p.6 / Chapter 1.1.2.2 --- Role of BKCa channels in the regulation of vascular function --- p.6 / Chapter 1.2 --- Functional alteration of the coronary SMCs during cardiac surgery --- p.7 / Chapter 1.2.1 --- Effect of ischemia on the function of SMCs in the coronary circulation --- p.8 / Chapter 1.2.2 --- Effect of cardioplegic/organ preservation solutions on the function of SMCs in the coronary circulation --- p.11 / Chapter Chapter 2. --- Materials and Methods --- p.14 / Chapter 2.1 --- Isometric force study in small coronary arteries --- p.14 / Chapter 2.1.1 --- Preparation of porcine small coronary arteries --- p.14 / Chapter 2.1.2 --- Experiment procedure --- p.15 / Chapter 2.1.2.1 --- Mounting of small coronary arteries --- p.15 / Chapter 2.1.2.2 --- Normalization procedure for small coronary arteries --- p.16 / Chapter 2.1.2.3 --- Precontraction and relaxation --- p.17 / Chapter 2.1.3 --- Data acquisition and analysis --- p.17 / Chapter 2.2 --- Patch-clamp electrophysiology --- p.18 / Chapter 2.2.1 --- Preparation of porcine coronary arteries --- p.18 / Chapter 2.2.2 --- Enzymatic dissociation of coronary arterial SMCs --- p.18 / Chapter 2.2.3 --- Primary cell culture --- p.19 / Chapter 2.2.4 --- Recording of BKca channel currents --- p.19 / Chapter 2.3 --- Statistical analysis --- p.21 / Chapter 2.4 --- Chemicals --- p.21 / Chapter Chapter 3. --- The Effect of Ischemia on BKCa channels in the Isolated SMCs of Coronary Arteries --- p.22 / Chapter 3.1 --- Abstract --- p.22 / Chapter 3.2 --- Introduction --- p.23 / Chapter 3.3 --- Experimental design and analysis --- p.25 / Chapter 3.3.1 --- Isometric force study in small coronary arteries --- p.25 / Chapter 3.3.2 --- Effect of ischemia on NS1619-induced relaxation in small coronary arteries --- p.26 / Chapter 3.3.3 --- Effect of ischemia on smooth muscle BKca channel currents --- p.27 / Chapter 3.3.3.1 --- Preparation of porcine coronary artery --- p.27 / Chapter 3.3.3.2 --- Enzymatic dissociation of coronary arterial SMCs --- p.27 / Chapter 3.3.3.3 --- Recording of BKCa channel currents --- p.27 / Chapter 3.3.4 --- Data acquisition and analysis --- p.28 / Chapter 3.4 --- Results --- p.28 / Chapter 3.4.1 --- Electrophysiological studies --- p.28 / Chapter 3.4.1.1 --- Effect of IBTX on the whole cell outward currents --- p.29 / Chapter 3.4.1.2 --- Effect of ischemia on the IBTX-sensitive BKca currents --- p.30 / Chapter 3.4.2 --- Relaxation studies --- p.30 / Chapter 3.4.2.1 --- Resting force --- p.30 / Chapter 3.4.2.2 --- U46619-induced contraction force --- p.31 / Chapter 3.4.2.3 --- Effect of IBTX on the NS1619-induced relaxation --- p.31 / Chapter 3.4.2.4 --- Effect of ischemia on the NS1619-induced relaxation --- p.31 / Chapter 3.5 --- Discussion --- p.32 / Chapter 3.5.1 --- Functional changes of the coronary smooth muscle BKca channels after ischemic exposure --- p.33 / Chapter 3.5.2 --- Role of BKca channels in SMCs during ischemia --- p.33 / Chapter 3.5.3 --- Clinical implications --- p.35 / Chapter Chapter 4. --- The Effect of Hyperkalemia on BKCa channels in the Isolated SMCs of Coronary Arteries --- p.41 / Chapter 4.1 --- Abstract --- p.41 / Chapter 4.2 --- Introduction --- p.42 / Chapter 4.3 --- Experimental design and analysis --- p.44 / Chapter 4.3.1 --- Isometric force study in small coronary arteries --- p.44 / Chapter 4.3.1.1 --- Effect of hyperkalemia on NS1619-mediated relaxation in small coronary arteries --- p.44 / Chapter 4.3.2. --- Effect of hyperkalemia on BKCa currents of SMCs --- p.45 / Chapter 4.3.2.1 --- Preparation of porcine coronary arteries --- p.45 / Chapter 4.3.2.2 --- Enzymatic dissociation of coronary arterial SMCs --- p.45 / Chapter 4.3.2.3 --- Recording of BKca channel currents --- p.46 / Chapter 4.3.3. --- Data acquisition and analysis --- p.46 / Chapter 4.4 --- Results --- p.47 / Chapter 4.4.1 --- Effect of hyperkalemia on the iberiotoxin-sensitive BKCa channel currents --- p.47 / Chapter 4.4.2 --- Relaxation studies --- p.48 / Chapter 4.4.2.1 --- Resting force --- p.48 / Chapter 4.4.2.2 --- U46619- and high K+-induced contraction force --- p.48 / Chapter 4.4.2.3 --- Effect of high K+ on the NS1619-induced relaxation --- p.48 / Chapter 4.4.2.4 --- Effect of IBTX on the NS1619-induced relaxation --- p.49 / Chapter 4.5 --- Discussion --- p.49 / Chapter 4.5.1 --- Role of BKCa channels in the isolated SMCs in hyperkalemic solution --- p.50 / Chapter 4.5.2 --- Functional changes of BKCa channels in coronary SMCs in hyperkalemia exposure --- p.51 / Chapter 4.5.3 --- Clinical implications --- p.52 / Chapter Chapter 5. --- General Discussion --- p.58 / Chapter 5.1 --- BKCa channels in porcine coronary SMCs --- p.59 / Chapter 5.2 --- Alteration of BKCa function related to ischemia in porcine coronary SMCs --- p.60 / Chapter 5.3 --- Alteration of BKCa function related to hyperkalemia in porcine coronary SMCs --- p.61 / Chapter 5.4 --- Limitation of the study --- p.62 / Chapter 5.5 --- Future investigations --- p.63 / Chapter 5.6 --- Conclusions --- p.63 / References --- p.66

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326441
Date January 2008
ContributorsHan, Jianguo., Chinese University of Hong Kong Graduate School. Division of Surgery.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xiv, 76 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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