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The antioxidant and hypolipidemic effect of conjugated linoleic acid (CLA).

Yeung Chi Hang, Thomas. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (leaves 126-146). / Abstracts in English and Chinese. / Table of content / ACKNOWLEDGEMENT --- p.i / ABSTRACT --- p.ii / LIST OF ABBREVIATION --- p.vi / TABLE OF CONTENTS --- p.vii / Chapter Chapter1 --- General Introduction / Chapter 1.1 --- INTRODUCTION --- p.1 / Chapter 1.2 --- FORMATION OF CLA --- p.1 / Chapter 1.3 --- OCCURRENCE OF CLA IN FOODS --- p.5 / Chapter 1.4 --- PHYSIOLOGICAL EFFECTS OF CLA --- p.8 / Chapter 1.4.1 --- Anticarcinogenic Effects of CLA --- p.8 / Chapter 1.4.2 --- Antiatherogenic Effect of CLA --- p.11 / Chapter 1.4.3 --- Antioxidant Effect of CLA --- p.12 / Chapter 1.4.4 --- CLA and Immune Response --- p.13 / Chapter 1.4.5 --- CLA and Body Composition --- p.14 / Chapter Chapter2 --- Protective Effect of CLA on Copper-Induced Human LDL Oxidation / Chapter 2.1 --- INTRODUCTION --- p.17 / Chapter 2.1.1 --- Oxidative Modification of LDL and Atherosclerosis --- p.20 / Chapter 2.1.1.1 --- Understanding of LDL --- p.20 / Chapter 2.1.1.2 --- Oxidative Modification of LDL --- p.20 / Chapter 2.1.1.3 --- Role of Oxidative Modified LDL in Atherogenesis --- p.23 / Chapter 2.1.2 --- Antioxidants and Atherosclerosis --- p.25 / Chapter 2.1.3 --- Measuring TBARS Formation as an in vitro Index to Monitor LDL Oxidation --- p.26 / Chapter 2.1.4 --- CLA and Atherogenesis --- p.27 / Chapter 2.2 --- OBJECTIVE OF THE PRESENT STUDY --- p.28 / Chapter 2.3 --- MATERIALS AND METHODS --- p.29 / Chapter 2.3.1 --- Human LDL Isolation --- p.29 / Chapter 2.3.2 --- LDL Oxidation --- p.30 / Chapter 2.3.3 --- Thiobarbituric Acid Reactive Substances (TBARS) Assay --- p.30 / Chapter 2.4 --- STATISTICS --- p.31 / Chapter 2.5 --- RESULTS --- p.32 / Chapter 2.5.1 --- Inhibitory Effect of BSA on Human LDL Oxidation --- p.32 / Chapter 2.5.2 --- Pro-oxidant Effect of LA on Human LDL Oxidation --- p.32 / Chapter 2.5.3 --- Inhibitory Effect of CLA on Human LDL Oxidation --- p.32 / Chapter 2.6 --- DISCUSSION --- p.37 / Chapter 2.6.1 --- Effect ofBSA on Copper-Induced LDL Oxidation --- p.37 / Chapter 2.6.2 --- Effect of LA on Copper-Induced LDL Oxidation --- p.38 / Chapter 2.6.3 --- Protective Effect of CLA on Copper-Induced Human LDL Oxidation --- p.39 / Chapter Chapter3 --- Hypolipidemic Activity of CLA / Chapter 3.1 --- INTRODUCTION --- p.42 / Chapter 3.1.1 --- Total Cholesterol and LDL Cholesterol --- p.42 / Chapter 3.1.2 --- Triglyceride (TG) --- p.44 / Chapter 3.1.3 --- Hypolipidemic Effect of CLA --- p.45 / Chapter 3.1.4 --- Golden Syrian Hamster as an Animal Model of Cholesterol Metabolism --- p.46 / Chapter 3.2 --- OBJECTIVES OF THE PRESENT STUDY --- p.48 / Chapter 3.3 --- MATERIALS AND METHODS --- p.48 / Chapter 3.3.1 --- LA and CLA --- p.49 / Chapter 3.3.2 --- Animals --- p.49 / Chapter 3.3.3 --- Experiment1 --- p.49 / Chapter 3.3.4 --- Experiment2 --- p.51 / Chapter 3.3.5 --- "Determination of Serum TC, HDL-Cholesterol (HDL-C) and TG" --- p.54 / Chapter 3.3.6 --- Lipid analysis of Liver and Adipose Tissue --- p.54 / Chapter 3.3.6.1 --- Lipid Extraction and Separation of Different Lipid Species --- p.54 / Chapter 3.3.6.2 --- Acid-Catalyzed Methylation of Fatty Acids --- p.55 / Chapter 3.3.6.3 --- GLC Analysis of FAME --- p.55 / Chapter 3.3.7 --- Quantification of Tissue Cholesterol --- p.56 / Chapter 3.3.7.1 --- Cholesterol Extraction and Silylation --- p.56 / Chapter 3.3.7.2 --- GLC Analysis of TMS-Ether Derivative of Cholesterol --- p.56 / Chapter 3.4 --- STATISTICS --- p.57 / Chapter 3.5 --- RESULTS --- p.59 / Chapter 3.5.1 --- Body Weight and Food Intake --- p.59 / Chapter 3.5.2 --- "Effect of Dietary CLA Supplementation on Serum TG, TC and HDL-C" --- p.59 / Chapter 3.5.3 --- "Effect of Dietary CLA Supplementation on Hepatic TG, Phospholipid and Cholesterol" --- p.64 / Chapter 3.5.4 --- Effect of Dietary CLA Supplementation on Adipose Tissue TG and Cholesterol --- p.73 / Chapter 3.5.5 --- Effect of CLA Supplementation on Cholesterol Levels of Different Tissues --- p.73 / Chapter 3.6 --- DISCUSSION --- p.79 / Chapter 3.6.1 --- "Effect of CLA Supplementation on Serum TG, TC and HDL-C" --- p.79 / Chapter 3.6.2 --- "Effect of CLA Supplementation on Hepatic TG, PL and Cholesterol" --- p.81 / Chapter 3.6.3 --- Effect of CLA on Adipose Tissue TG and Cholesterol --- p.83 / Chapter 3.6.4 --- Implication of CLA Intake in Humans --- p.84 / Chapter Chapter4 --- Influences of Dietary CLA on Cholesterol Homeostasis / Chapter 4.1 --- INTRODUCTION --- p.86 / Chapter 4.2 --- NEUTRAL EFFECT OF DIETARY CLA SUPPLEMENTATION ON HMG-COA REDUCTASE ACTIVITY --- p.88 / Chapter 4.2.1 --- HMG-CoA Reductase as the Rate-Limiting Enzyme in Cholesterol Synthesis --- p.88 / Chapter 4.2.2 --- Objective of The Present Study --- p.91 / Chapter 4.2.3 --- Materials and Methods --- p.92 / Chapter 4.2.3.1 --- Preparation of Hepatic Microsome --- p.92 / Chapter 4.2.3.2 --- HMG-GoA Reductase Activity Assay --- p.92 / Chapter 4.2.4 --- Statistics --- p.93 / Chapter 4.2.5 --- Results --- p.94 / Chapter 4.2.6 --- Discussion --- p.96 / Chapter 4.3 --- DOWN-REGULATION OF THE INTESTINAL ACAT ACTIVITY BY CLA FEEDING --- p.97 / Chapter 4.3.1 --- Role of ACAT in Cholesterol Absorption --- p.97 / Chapter 4.3.2 --- Objective of The Present Study --- p.99 / Chapter 4.3.3 --- Materials and Methods --- p.100 / Chapter 4.3.3.1 --- Preparation of Intestinal Microsome --- p.100 / Chapter 4.3.3.2 --- ACAT Activity Assay --- p.100 / Chapter 4.3.4 --- Statistics --- p.101 / Chapter 4.3.5 --- Results --- p.102 / Chapter 4.3.6 --- Discussion --- p.104 / Chapter 4.4 --- ALTERATION OF FECAL EXCRETION BY DIETARY CLA --- p.105 / Chapter 4.4.1 --- Objective of The Present Study --- p.108 / Chapter 4.4.2 --- Materials and Methods --- p.109 / Chapter 4.4.2.1 --- Separation of Neutral and Acidic Sterols --- p.109 / Chapter 4.4.2.2 --- Neutral Sterol Analysis --- p.109 / Chapter 4.4.2.3 --- Acidic Sterol Analysis --- p.110 / Chapter 4.4.2.4 --- GLC Analysis of Neutral and Acidic Sterols --- p.110 / Chapter 4.4.3 --- Statistics --- p.113 / Chapter 4.4.4 --- Results --- p.114 / Chapter 4.4.4.1 --- Effect of CLA Supplementation on Fecal Output of Neutral Sterols --- p.114 / Chapter 4.4.4.2 --- Effect of CLA Supplementation on Fecal Output of Acidic Sterols --- p.114 / Chapter 4.4.5 --- Discussion --- p.118 / Chapter Chapter5 --- Conclusions --- p.123 / References --- p.126

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_322906
Date January 1999
ContributorsYeung, Chi Hang Thomas., Chinese University of Hong Kong Graduate School. Division of Biochemistry.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, x, 146 leaves : ill. (some col.) ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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