This study evaluates the efficiency of albendazole against different stages of Angiostrongylus cantonensis in Sprague-Dawley rats. Using a single oral dose of albendazole of 10 mg/kg at 0 or 1 or 2 or 3 weeks post-infection (PI) showed that the drug in 0 or 1 weeks PI was more effective in the larval infection. Compared to those in non-treated control, the rats treated 0 or 1 weeks PI showed significant reductions not only in the relative wet weight of heart and lungs (g/100 g body weight), but also in the mean number of adults recovered. With regard to single oral doses of 10 or 50 or 100 mg/kg at weekly intervals from 6 weeks PI, the rats treated 100 mg/kg in 2 weeks post-treatment (PT) showed significant reduction in the L1 in its feces and the amounts were lastingly few. In the rats treated 10 or 50 mg/kg, larval output reappeared from 4 weeks PT. The histomorphology of granuloma formation was also investigated in the lungs of SD rats infected with A. cantonensis¡@by paraffin section. It was observed that granulomatous fibros was strongly associated with the number of L1 in the lungs.
IgG and IgE antibodies specific for antigens from L1, L5, male worms or female adults of A. cantonensis were respectively measured by an enzyme-linked immunosorbent assay (ELISA). The specific IgE response to antigens from L1 or female adults of A. cantonensis in the serum reached the maximum at 1 and 5 weeks PI. Similarly, the eosinophil response also revealed remarkable peaks at 1 and 5 weeks PI. These results might prove that the increases of IgE titer and eosinophil are related to antibody-dependent cell mediated cytotoxicity (ADCC).
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0715105-133726 |
| Date | 15 July 2005 |
| Creators | Lai, Pang-Yueh |
| Contributors | none, none, none |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0715105-133726 |
| Rights | unrestricted, Copyright information available at source archive |
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