The present study used Escherichia coli¡]BL21¡^that contained pET-23a-bpGH plasmids, to express black porgy growth hormone ¡]bpGH¡^. The bpGH was refolded at pH 11.3 in the presence of catalytic amounts of cysteine and purified by ion exchanger chromatography and gel filtration chromatography. The purified bpGH is a monomer and has a molecular weight of 22 kDa. Using the bpGH, the effects of the growth hormone on growth, essential amino acid deposition and nonspecific immunity in black porgy were studied. The experiment was a 4*3 ¡]diet*GH¡^ factorial design. Four experimental diets were formulated in that fish meal was replaced by the mixture of soya protein and gelatin so that fish meal / soya mixture = 100 / 0, 75 / 25, 50 / 50 and 25 / 75, respectively. GH treatments included non-injection, once per 3 days and once per 12 days. GH was injected intramuscularly at a dosage of 0.05 µg / g wet body weight. The growth trial lasted for 72 days. The results showed that GH administration significantly enhanced weight gain, feed efficiency, protein efficiency ratio and muscle methionine concentration of the fish. GH injection improved the growth performance of the fish fed low protein quality diets to a level equals to the groups fed high protein quality diets. These results indicate that GH injections enhanced the perferential absorption and deposition of the first limiting amino acid methionine from the diets. In addition, GH administration enhanced alternative complement activity and increased serum lysozyme concentration, implicating the enhancement of the immunity.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0706100-141923 |
| Date | 06 July 2000 |
| Creators | Doong, Jaan-Rong |
| Contributors | Houng-Yung Chen, I-Chin Liao, Chen-Chin Kao, Shi-Yen Shiau |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0706100-141923 |
| Rights | unrestricted, Copyright information available at source archive |
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