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Mikrobiální společenstva a metagenom průmyslově znečištěných půd: výskyt genů kódujících AEH / Microbial consortia and metagenome of industrially polluted soil: occurrence of genes encoding AEH

Soils contain highly diverse consortia of bacteria making them very attractive starting points for both culture-dependent and metagenomic discovery efforts. The present diploma thesis analyses the composition of the microbial community from pharmaceutically polluted soil, with the employment of next-generation Illumina sequencing of 16S rDNA region. This analysis revealed high complexity of the soil microbial environment and confirmed that anthropogenic activity (represented by production of beta- lactam antibiotics) influences the variability and abundance of the species, yet without reducing the microbial diversity. In the second part of the thesis, isolation and heterologous expression of a novel gene encoding alpha-amino acid ester hydrolase (AEH) from a cultivable soil microorganism B. cereus is described. AEHs possess industrial potential for biocatalytic synthesis of semi-synthetic beta-lactam antibiotics, which are presently of great clinical importance. Powered by TCPDF (www.tcpdf.org)

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:331139
Date January 2015
CreatorsPitkina, Anastasiya
ContributorsKyslík, Pavel, Lichá, Irena
Source SetsCzech ETDs
LanguageEnglish
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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