While a decrease of wild felid population has led to disruption of conservation
programme, recent studies have shown the importance of immune regulation for
determining health outcomes and co-infection. Immunoglobulin G is important for
detecting and evaluating responses to infectious diseases and vaccination. But, there
is limited information on felid immunoglobulins and their role for functional immunity.
This study aimed at isolating and characterizing lion’s immunoglobulin G. Lions’ sera
(n = 68) were processed using the MagReSyn® magnetic beads and the final protein
concentration was determined using the Xpose™ Trinean Spectrophotometer. The
cross-reactivity of goat anti-cat immunoglobulin with sera of lions and other species
was analysed using ELISA. High cross-reactivity was observed in lions ranging from
87.7 to 100%, and low reactivity with rhino (22.4%) followed by chicken (0.01%). The
protein concentration from purified sera yielded 39.09 mg/ml. Molecular weight of lion
IgG 150-160 kDa was detected with both chains at 54-56 kDa and 24-26 kDa on SDS PAGE. These results indicate a potential aid in developing serological tools to monitor
exposure to micro-organisms of lions. / Agriculture and Animal Health / M. Sc. (Agriculture)
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:unisa/oai:uir.unisa.ac.za:10500/27525 |
| Date | 06 1900 |
| Creators | Manamela, Tebogo Sabina |
| Contributors | Kayoka, P. N., Morar-Leather, D. |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Dissertation |
| Format | 1 online resource (68 leaves) : illustrations (some color), graphs (some color), color map, application/pdf |
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