Disturbing the interaction between sympathetic nerves and macrophages in islets has been shown to protect from insulitis development. To study the intra-islet neuroimmune communications, we explored the interactions between macrophages and nerves in the islets by looking at their juxta-positioning in the islets of wild type and CX3CR1 knock out mice. We also cultured M1/M2 bone marrow-derived macrophages in order to understand basic expression profile changes during catecholaminergic stimulation. The results showed weaker colocalization between intra-islet macrophages and sympathetic nerves in CX3CR1-deficient mice as compared to the wild type showing that CX3CR1 played an important role in nerve-macrophage interactions. Also, low concentration of norepinephrine might induce pro-inflammatory effects in M1, as implied in how intra-islet macrophages responded to nerve signal excitation in type 1 diabetes development.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-442377 |
| Date | January 2021 |
| Creators | Wang, Yuexi |
| Publisher | Uppsala universitet, Institutionen för medicinsk cellbiologi, Uppsala universitet, Institutionen för biologisk grundutbildning |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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