The effects of gold sodium thiomalate (GST) on murine spleen cells were investigated using in vitro mitogen blastogenesis techniques. Addition of GST to intact spleen cells resulted in a decreased blastogenic response to the T cell mitogen, concanavalin A (Con A). Thymidine uptake of spleen cells depleted of macrophages and cultured with Con A and GST demonstrated biphasic effects. At 2.5 pg Con A/ml, blastogenesis of macrophage depleted spleen cells was inhibited to a lesser degree than intact spleen cells; whereas, at 0.5 pg Con A/ml, the macrophage depleted spleen cells were inhibited to a greater degree than the intact spleen cells. Addition of GST at intervals ranging from 0 to 48 hours indicated that inhibition occurred within 36 hours following mitogen stimulation. These results suggest that GST inhibits early events of lymphocyte activation by direct interaction with lymphocytes.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc500436 |
| Date | 12 1900 |
| Creators | Brownback, Paul (Paul Eldon) |
| Contributors | Measel, John William, Jr., Barrow, William W., Goven, Arthur James, 1950- |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | vi, 71 leaves: ill., Text |
| Rights | Public, Brownback, Paul (Paul Eldon), Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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