Tyrosine tRNA was isolated from bovine liver and its nucleotide sequence was determined using in vitro 32p_ labeling techniques. Several important structural features of the tRNA are: the presence of gal-Q in the first position of the anticodon, acp3U at position 20, and a pair of adjacent N,N-dimethylguanosines (residues 26 and 27). A human DNA fragment harbored in a lambda phage clone was isolated, and restriction enzyme analysis revealed the presence of three tRNA genes in a 6.0-kb BamHI subfragment. Portions of the 6.0-kb DNA fragment containing the tRNA genes were sequenced by the method of Maxam and Gilbert and analyzed for transcriptional activity in vitro using homologous cytoplasmic extracts. A threonine tRNAUGU gene exhibited high transcriptional activity dependent on its 5'- flanking sequence. The enhanced transcription is not completely inhibited by alpha-amanitin. The value of studying tRNA structure in concert with the cognate tRNA. genes is discussed.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc500631 |
| Date | 08 1900 |
| Creators | Johnson, Gary D. (Gary Dean), 1960- |
| Contributors | Pirtle, Robert M., Norton, S. J., Wu, Edward Ming-chi, 1938- |
| Publisher | North Texas State University |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | ix, 88 leaves: ill., Text |
| Rights | Public, Johnson, Gary D. (Gary Dean), 1960-, Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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