In situ hybridization (ISH) has played a crucial role in developing a spatial transcriptomic
understanding of emerging model organisms in the past, but advancing high-throughput
RNA-sequencing (RNA-seq) technology has pushed this method into the shadows, leading
to a loss of data resolution. This shift in research towards the exclusive use of RNA-seq
neglects essential considerations for transcriptomic studies including the spatial and
temporal expression of transcripts, available budget, experimental design needs, and
validation of data. A synergy of spatiotemporal transcriptomic techniques is needed,
using the bulk and unbiased analysis of RNA-seq and the visual validation and
spatiotemporal resolution of ISH. Integration of this synergistic approach can improve our
molecular understanding of non-model organisms and establish the background data
needed for advancing research techniques. A prime example lies within an emerging
model of the marine science and symbiosis fields, where I present a case study on a
threatened coral reef keystone – the cnidarian-dinoflagellate symbiosis. Establishing a
whole-mount ISH protocol for the emerging cnidarian model Aiptasia (sea anemone) will
help future studies reveal the gene regulation underpinning the establishment,
persistence, and breakdown of this complex symbiotic relationship.
| Identifer | oai:union.ndltd.org:kaust.edu.sa/oai:repository.kaust.edu.sa:10754/670220 |
| Date | 07 1900 |
| Creators | Watson, Kelly |
| Contributors | Aranda, Manuel, Biological and Environmental Science and Engineering (BESE) Division, Peixoto, Raquel S, Frøkjær-Jensen, Christian |
| Source Sets | King Abdullah University of Science and Technology |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Rights | 2022-07-14, At the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis will become available to the public after the expiration of the embargo on 2022-07-14. |
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