Functional inactivation of tumor susceptibility gene tsg101 leads to cellular transformation and tumorigenesis in mice. No genomic DNA deletion in TSG101gene in human cancer indicated TSG101 is not a typical tumor suppressive gene. TSG101 participates in the MDM2/p53 feedback control loop and the regulation of the cellular membrane trafficking. However, detail functional characteristics remains to be elucidate.
In this study, we explored the tsg101 expression in adult mouse tissues from various organs using immunohistochemistry and in situ hybrid- ization. The results indicated that tsg101 expression was ubiquitous but in differential steady-state level in various cell types. The expression of tsg101 mainly found in epithelial cells¡Bsecretory cells and nerve cells. The second topic of this study was to characterize the phosphorylation status of TSG101 protein. Endogeneously expressed TSG101 and exogeneously expressed HA-tag TSG101 protein were purified by immunoprecipiation with #820 antiserum against TSG101, and were subjected for western blot analysis using anti-phosphoserine and anti-phosphothreonine antibodies. This experiment had confirmed that TSG101 protein contained both phosphoserine and phosphthreonine residues. In vitro kianse assay using GST-tag and his-tag TSG101 funsion proteins was exploited to investigate the kinase responsible for TSG101 phosphorylation. The results clearly indicated that cdc2¡BGSK3£] and PKC kinases could phosphorylate TSG101 fusion Protein, implying that the function of TSG101 might be regulated by the signaling involving these kinases.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0708103-151602 |
| Date | 08 July 2003 |
| Creators | Tsai, Hong-Yuan |
| Contributors | P-J, Lu, Yi-Ren Hong, Hung-Tu, Huang,, Jiin-Tsuey, Cheng |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0708103-151602 |
| Rights | withheld, Copyright information available at source archive |
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