Glyoxylase II (Glo II, E.C. 3.1.2.6) catalyzes the hydrolysis of S-D-Lactoylglutathione (SLG) to D-Lactate and glutathione. This is the rate limiting step in the conversion of methylglyoxal to D-Lactate. The purpose of the present study was to determine whether or not a relationship exists between some naturally occuring metabolites and in vivo modulation of Glo II. We have observed a non-competitive inhibition (~ 45%) of Glo II in crude preparation of rat liver by GTP (0.3 mM). A factor (apparently protein),devoid of Glo II,when reconstituted with the purified Glo II, enhanced Glo II activity. This coordinate activation and inhibition of Glo II suggest a mechanism whereby SLG levels can be modulated in vivo.
| Identifer | oai:union.ndltd.org:unt.edu/info:ark/67531/metadc500992 |
| Date | 08 1900 |
| Creators | Mbamalu, Godwin E. |
| Contributors | Norton, S. J., Harris, Ben G., Wu, Edward Ming-chi, 1938- |
| Publisher | University of North Texas |
| Source Sets | University of North Texas |
| Language | English |
| Detected Language | English |
| Type | Thesis or Dissertation |
| Format | v, 48 leaves: ill., Text |
| Rights | Public, Mbamalu, Godwin E., Copyright, Copyright is held by the author, unless otherwise noted. All rights reserved. |
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