Many functions in host–microbiota interactions are potentially influenced by intestinal
transit times, but little is known about the effects of altered transition times on the composition
and functionality of gut microbiota. To analyze these effects, we cultivated the model community
SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the
community structure and function. After five days of continuous cultivation, we investigated the
influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community,
we applied flow cytometric fingerprinting and revealed changes in the community structure of
SIHUMIx during the change of TT, which were not associated with changes in species abundances.
For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found,
along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid
metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and
isovalerate concentrations. Specifically, B. thetaiotaomicron was identified to be affected in terms of
butyrate metabolism. However, communities could recover to the original state afterward. This study
shows that SIHUMIx showed high structural stability when TT changed—even four-fold. Resistance
values remained high, which suggests that TTs did not interfere with the structure of the community
to a certain degree.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:84650 |
| Date | 11 April 2023 |
| Creators | Schäpe, Stephanie Serena, Krause, Jannike Lea, Engelmann, Beatrice, Fritz-Wallace, Katarina, Schattenberg, Florian, Liu, Zishu, Müller, Susann, Jehmlich, Nico, Rolle-Kampczyk, Ulrike, Herberth, Gunda, von Bergen, Martin |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2076-2607, 641 |
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