<p> The mitochondrial inner membrane is highly selective with regard
to permeability to solutes and the movement of a large number of large
or charged molecules across it therefore requires specific transport
processes provided by specific membrane proteins. </p> <p> In order to study the spatial arrangement of one such protein
the adenine nucleotide translocator protein which transports ADP and ATP
across the mitochondrial inner membrane, a number of chemical labelling
studies of the mitochondrial inner membrane were carried out. </p> <p> Mitochondrial inner membrane preparations of normal (mitoplasts)
and inverted (submitochondrial particles) config~ration with respect to
mitochondria have been isolated and the external phosphatidylethanolamine
and proteins modified by 3H isethionyl acetimidate. An upper limit of
40-46% of the total PE in mitoplasts was found to be located in the
external monolayer. </p> <p> Differences in protein labelling patterns of isethionyl acetimidate modified mitochondria and SMP was observed. JAI was found to penetrate the
outer membrane but not the inner membrane of intact mitochondria. </p> <p> A tritiated photoreactive phospholipid, 1 palmitoyl-2-(mdiazirinophenoxynonanoyl)
phosphatidylcholine (DAP-PC) was incorporated
into mitoplasts and submitochondrial particles symmetrically into both
monolayers by sonication and asymmetrically using phospholipid exchange
protein isolated from beef heart. Photolysis yielded the translocator as a major crosslinked product in both types of particles and with both
methods of incorporation. </p> <p> It was shown that the adenine nucleotide translocator can be asymmetrically labelled by modification of membrane.particles of
opposite orientation by water soluble and membrane soluble probes. </p> / Thesis / Master of Science (MSc)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/20786 |
| Date | 09 1900 |
| Creators | Wrona, Lynne |
| Contributors | Gerber, G. E., Biochemistry |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
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