The studies described in this thesis have used principally the rat neonatal cardiomyocyte (NCM) model to investigate previously unresolved questions regarding inositol phosphate signalling in the heart. Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) is known to be an arrhythmogenic molecule in the setting of cardiacischaemia and subsequent reperfusion, but the mechanisms responsible for its enhanced generation in pathological circumstances, as well as those suppressing its generation during phospholipase C (PLC)-coupled receptor stimulation under physiological conditions, have not been characterised. [3H]Inositol-labelling in combination with anion-exchange high performance liquid chromatography (HPLC)was used to gain an accurate picture of the changes in various [3H]InsP isomers induced by PLC stimulation.
| Identifer | oai:union.ndltd.org:ADTP/245088 |
| Creators | Matkovich, Scot J. |
| Source Sets | Australiasian Digital Theses Program |
| Language | English |
| Detected Language | English |
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