Previous studies have shown growth hormone and therapeutic low intensity pulsed
ultrasound can enhance mandibular growth separately. The aim of this study is to evaluate the
concomitant effect of both of these applications on mandibular growth in rat. Methods: 24 male
Sprague Dawley rats were divided into four groups, 6 in each. Groups 1, 2, 3, and 4 were
designated as untreated control, recombinant rat growth hormone, Low Intensity Pulsed
Ultrasound, and combination of both groups respectively. After 21 days of daily treatment on
mandibular condylar, mandibles from euthanized rats are dissected, and scanned by MicroComputed Tomography to measure the mandibular bone volume, bone surface area, and condylar
bone mineral density. Also Real-time Polymerase Chain Reaction was performed on the extracted
livers’ C-fos, C-jun, and IGF-1 genes expressions. Results: Groups 2, 3 and 4 showed significant
(p<0.05) growth stimulation when compared to the untreated control group. However, there was
no statistical significant difference between groups 2, 3 and 4 with regard to bone volume or
surface area. Conversely, condylar bone mineral density for group 4 was significantly reduced
than groups 1, 2, and 3. Rats’ weights were not significantly different among the treatment groups
after the treatment was performed. Additionally, gene expression study showed that the expression
of C-jun, in harvested livers for Group 4 was less than that of Group 2 showing fewer side effects.
Conclusion: When growth hormone was applied to rats’ mandible together with therapeutic
ultrasound, preferential increase in bone volume, and surface area occurred with the expense of
condylar bone mineral density and with less potential side effects. / Pharmaceutical Science
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:AEU.10048/1698 |
| Date | 06 1900 |
| Creators | Khan, Imran |
| Contributors | El-Kadi, Ayman, El-Bialy, Tarek, Dederich, Douglas, Siraki, Arno |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 786575 bytes, application/pdf |
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