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Možnosti trojrozměrného zobrazování v transmisním holografickém mikroskopu / Possibilities of three-dimensional imaging in transmitted-light holographic microscope

Digital holographic microscopy (DHM) is noninvasive method for obtaining images even from samples with low contrast. Nowadays DHM design makes it possible to illuminate sample by broad light source, halogene lamp. Broad light source is displayed in the front focal plane of condensor in such way, that Köhler illumination is achieved. Each point of the source corresponds to a plane wave in image field of objective, which illuminates the whole field of view, but from different direction. Position of the point determines the direction of illumination. In this reason, the microscope enables so reconstruct not only intensity, but also phase of object wave. New designed and constructed interchangeable pinhole aperture modify broad light illumination by rotation around the optical axis. Aperture is placed eccentrically considering the optical axis of microscope. Incidence of light beam on a sample would be under defined angle. After the reconstruction of taken phase images from individual angles of illumination should be possible to obtain three-dimensional structure of the sample.

Identiferoai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:230610
Date January 2013
CreatorsSládková, Lucia
ContributorsJákl, Petr, Kolman, Pavel
PublisherVysoké učení technické v Brně. Fakulta strojního inženýrství
Source SetsCzech ETDs
LanguageCzech
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/masterThesis
Rightsinfo:eu-repo/semantics/restrictedAccess

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