Angiotensin II (All) was found to upregulate vascular endothelial growth factor (VEGF) gene expression in rat heart and mesenteric artery-derived endothelial cells in a dose and time-dependent manner. Maximal induction was achieved by 2 h after the addition of All, Addition of cyctoheximide superinduced and actinomycin D abolished the induction. In addition, the induction could be blocked by tosartan, an AT1 receptor antagonist and protein kinase C inhibitors, H7 and GF109203X. These results suggest that induction of VEGF transcripts by All is dependent on protein kinase C dependent pathway and de novo RNA synthesis. Immunoblot analysis revealed that VEGF was detectable in the conditioned medium 8 h after All stimulation. Our data suggest that All-induced VEGF plays a role in the change of endothelial cell permeability and angiogenesis in renovascular hypertension (Supported by Cécile Cox Quillen Chair of Geriatrics).
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etsu-works-14755 |
| Date | 01 December 1996 |
| Creators | Chua, G. G., Hamdy, R. C., Chua, B. H.L. |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Source | ETSU Faculty Works |
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