N5-59, a siderophore deficient mutant strain of Rhodococcus erythropolis strain IGTS8 (IGTS8) was investigated to learn more about how this poorly characterized bacterium acquires iron. N5-59 cells were starved for iron and then lysed to release any intracellular siderophore. No intracellular siderophore was detected indicating that N5-59 is not defective in the export or release of siderophore but is probably unable to synthesize siderophore. In a cross-feeding bioassay, growth of N5-59 (in an iron depleted medium) was greatly enhanced by the addition of exogenous siderophore from IGTS8 and other Rhodococcus species indicating that N5-59 is not defective in siderophore uptake. A DNA hybridization probe was prepared using DNA flanking the site of insertional mutation in strain N5-59. This probe was then used to clone a 6 kilobase pair, PstI restriction fragment from the chromosome IGTS8. This cloned DNA is expected to contain the intact gene(s) that was interrupted in N5-59.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:etd-2002 |
| Date | 13 December 2003 |
| Creators | Moretz, Samuel Eugene |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Electronic Theses and Dissertations |
| Rights | Copyright by the authors. |
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