Yu Yun Lung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2000. / Includes bibliographical references (leaves 101-114). / Abstracts in English and Chinese. / Acknowledgments / Abstract / Table of Contents / Chapter CHAPTER 1 --- GENERAL INTRODUCTION --- p.1 / Chapter 1.1 --- General Structure of Lectins --- p.1 / Chapter 1.1.1 --- Metal Binding Sites --- p.2 / Chapter 1.1.2. --- Hydrophobic Sites --- p.3 / Chapter 1.1.3. --- Glycosylation Sites --- p.3 / Chapter 1.2 --- Carbohydrate Specificities of Lectins --- p.4 / Chapter 1.3 --- Plant Lectins --- p.4 / Chapter 1.3.1 --- Localization of lectins in plants --- p.4 / Chapter 1.3.1.1 --- Localization in seeds --- p.4 / Chapter 1.3.1.2 --- Localization in vegetative parts --- p.5 / Chapter 1.3.1.3 --- Biosynthesis of plant lectins --- p.6 / Chapter 1.3.2 --- Functions of plant lectins in plants --- p.7 / Chapter 1.3.2.1 --- In cell growth --- p.7 / Chapter 1.3.2.2 --- In storage --- p.8 / Chapter 1.3.2.3 --- In plant defence --- p.8 / Chapter 1.3.2.4 --- In nitrogen cycle --- p.10 / Chapter 1.3.3 --- Biological activities of plant lectins in other organisms --- p.13 / Chapter 1.3.3.1 --- Immunomodulatory activity --- p.13 / Chapter 1.3.3.2 --- Antitumor and antiproliferative activities --- p.14 / Chapter 1.3.3.3 --- Mitogenic activity --- p.14 / Chapter 1.3.3.4 --- Antiviral activity --- p.14 / Chapter 1.3.4 --- Relationship between lectins and ribosome inactivating proteins: family of ricin-related proteins --- p.16 / Chapter 1.3.5 --- Applications of plant lectins --- p.18 / Chapter 1.3.5.1 --- In scientific research --- p.18 / Chapter 1.3.5.2 --- In medical research --- p.19 / Chapter 1.4 --- Animal Lectins --- p.20 / Chapter 1.4.1 --- Some properties of animal lectins --- p.20 / Chapter 1.4.2 --- Functions of animal lectins --- p.22 / Chapter 1.4.2.1 --- In protein metabolism --- p.22 / Chapter 1.4.2.2 --- As a mediator of binding and phagocytosis of microorganisms --- p.22 / Chapter 1.4.2.3 --- Control of differentiation and organ formation --- p.23 / Chapter 1.4.2.4 --- Lectins and migration of lymphocytes --- p.23 / Chapter 1.4.2.5 --- Lectins and metastasis --- p.24 / Chapter 1.5 --- Mushroom lectins --- p.25 / Chapter 1.6 --- Regulation of lectins --- p.29 / Chapter 1.7 --- Isolation and purification of lectins --- p.31 / Chapter 1.8 --- Objectives of the present study --- p.33 / Chapter CHAPTER 2 --- "SCREENING FOR HEMAGGLUTINATING ACTIVITY IN EXTRACTS OF SEEDS, FRUITS, VEGETABLES AND CHINESE MEDICINAL HERBS" --- p.35 / Chapter 2.1 --- Introduction --- p.35 / Chapter 2.2 --- Materials and methods --- p.36 / Chapter 2.3 --- Results --- p.38 / Chapter 2.4 --- Discussion --- p.38 / Chapter CHAPTER 3 --- ISOLATION OF LECTIN FROM RHIZOMES OF SMILAX GLABRA (FAMILY LILIACEAE) --- p.43 / Chapter 3.1 --- Introduction --- p.43 / Chapter 3.1.1 --- Introduction about Smilax glabra and its chemical constituents --- p.43 / Chapter 3.1.2 --- Introduction about monocot lectins including Liliaceae lectins --- p.45 / Chapter 3.2 --- Materials and methods --- p.50 / Chapter 3.2.1 --- Isolation of lectins from Smilax glabra rhizomes --- p.50 / Chapter 3.2.2 --- Assay for hemagglutinating activity --- p.55 / Chapter 3.2.3 --- Test of inhibition of lectin-induced hemagglutination by various carbohydrates --- p.55 / Chapter 3.2.4 --- "Effects of acid, alkali, temperature and cations on hemagglutinationg activity of lectin" --- p.56 / Chapter 3.2.5 --- Determination of protein concentration --- p.56 / Chapter 3.2.6 --- Molecular mass determination by SDS-PAGE --- p.56 / Chapter 3.2.7 --- Molecular mass determination by gel filtration --- p.56 / Chapter 3.2.8 --- Amino acid sequence analysis --- p.57 / Chapter 3.3 --- Results --- p.57 / Chapter CHAPTER 4 --- ISOLATION OF LECTIN FROM SEEDS OF THE CHINESE CHESTNUT CASTANEA MOLLISIMA (FAMILY FAGACEAE) --- p.74 / Chapter 4.1 --- Introduction to Castanea mollisima and its chemical constituents --- p.74 / Chapter 4.2 --- Materials and Methods --- p.78 / Chapter 4.2.1 --- Isolation of lectin from Chinese chestnuts --- p.78 / Chapter 4.2.2 --- Assay for hemagglutinating activity --- p.83 / Chapter 4.2.3 --- Test of inhibition of lectin-induced hemagglutination by various carbohydrates --- p.83 / Chapter 4.2.4 --- "Effects of acid, alkali, temperature and cations on hemagglutinationg activity of lectin" --- p.83 / Chapter 4.2.5 --- Determination of protein concentration --- p.83 / Chapter 4.2.6 --- Molecular mass determination by SDS-PAGE --- p.83 / Chapter 4.2.7 --- Molecular mass determination by gel filtration --- p.83 / Chapter 4.2.8 --- Amino acid sequence analysis --- p.83 / Chapter 4.3 --- Results --- p.84 / Chapter 4.4 --- Discussion --- p.96 / Chapter CHAPTER 5 --- GENERAL DISCUSSION AND CONCLUSION --- p.98 / REFERENCES: --- p.101
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_323160 |
| Date | January 2000 |
| Contributors | Yu, Yun Lung., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, ix, 114 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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